Comparison of the Prevention of Aflatoxin B1-Induced Genotoxicity by Quercetin and Quercetin Pentaacetate
a Department of Biochemistry, V. P. Chest Institute, University of Delhi, Delhi-110 007, India
b Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi-110 007, India
Received 22 October 2001; accepted 20 June 2002. Available online 15 August 2002.
Earlier work carried out
in our laboratory highlighted the mode of action of acetoxy
4-methylcoumarins in preventing the genotoxicity of aflatoxin B1
(AFB1). We have in this report extended the observations to
quercetin pentaacetate (QPA), which unlike quercetin (Q) has demonstrated
time-dependent inhibition of liver microsome catalysed AFB1
epoxidation as measured by AFB1 binding to DNA. The action of
QPA is similar to that of the acetoxy 4-methylcoumarins in that they are
acted upon by microsomal transacetylase leading to modulation of catalytic
activities of certain enzymes (such as P-450 enzymes, NADPH cytochrome C
reductase and glutathione S-transferase) possibly by way of protein
acetylation. In the present work, we have documented the transacetylase-mediated
action of QPA in preventing genotoxicity due to AFB1.
Quercetin penta acetate (QPA)
unlike quercetin demonstrated time-dependent inhibition of liver microsome-catalyzed
AFB1 epoxidation as measured by AFB1 binding to DNA.
In the present work, we have demonstrated the transacetylase-mediated
action of QPA in the prevention of genotoxicity due to AFB1.
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